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Authentication of the family Polygonaceae in Chinese pharmacopoeia by DNA barcoding technique
- Source :
-
Journal of Ethnopharmacology . Jul2009, Vol. 124 Issue 3, p434-439. 6p. - Publication Year :
- 2009
-
Abstract
- Abstract: Ethnopharmacological relevance: Medicinal plants belonging to the family Polygonaceae in Chinese pharmacopoeia possess important medicinal efficacy in traditional Chinese medicines. Aim of the study: DNA barcodes are first used to discriminate the Polygonaceae in Chinese pharmacopoeia and their adulterants. Materials and methods: DNA samples, extracted from thirty-eight specimens belonging to eighteen species in Polygonaceae, were used as templates. Eight candidate barcodes were amplified by polymerase chain reaction. Sequence analysis was accomplished by CodonCode Aligner V 2.06 and DNAman V 6. Species identification was performed using MEGA V 4.0. Results: The amplification efficiency of six candidate DNA barcodes (rbcL, trnH-psbA, ndhJ, rpoB, rpoC1, accD) was 100%, while the efficiency of YCF5 and nrITS was 56% and 44%, respectively. The interspecific divergence was highest for the trnH-psbA (20.05%), followed by the nrITS (14.01%) across all species pairs, while intraspecific variation both within populations and between populations was absent (0.0%). The trnH-psbA can not only distinguish ten species of Polygonaceae in Chinese pharmacopoeia, but also recognize eight other species of Polygonaceae including their adulterants. Conclusion: Our findings show that DNA barcoding is an efficient tool for identification of Polygonaceae in Chinese pharmacopoeia and their adulterants. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 124
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 43470137
- Full Text :
- https://doi.org/10.1016/j.jep.2009.05.042