Antioxidant and anticancer activities of 8-hydroxypsoralen isolated from wampee [Clausena lansium (Lour.) Skeels] peel

Abstract: Fruits of wampee [Clausena lansium (Lour.) Skeels] contain a significant amount of coumarins with many health benefits. The activity-guided separation of an ethyl acetate-soluble fraction on a polyamide column followed by silica gel column and high performance liquid chromatography (HPLC) preparation afforded a pure compound, which was identified to be 8-hydroxypsoralen based on the 1H, 13C NMR (nuclear magnetic resonance), and ESI-MS (electrospray ionisation mass spectrometric) analysis. This isolate exhibited good scavenging activities against DPPH radical and superoxide anion as well as significant reducing power. It also showed potent proliferation inhibitory activity against human hepatocellular liver carcinoma cell line (HepG2), human lung adenocarcinoma epithelial cell line (A549) and human cervical carcinoma cell line (HELA). This is the first report on the antioxidant and cytotoxic properties of C. lansium fruit extract. The food and pharmaceutical industry could be benefited by the usage of this extract containing this constituent. [Copyright &y& Elsevier]