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Assessment of the impact of solvent/detergent treatment on the quality and potency of a whole IgG equine antivenom
- Source :
-
Biologicals . Oct2009, Vol. 37 Issue 5, p306-312. 7p. - Publication Year :
- 2009
-
Abstract
- Abstract: We have evaluated for the first time the impact of a solvent/detergent (S/D) treatment on the quality and in vivo neutralization potency of horse-derived whole IgG antivenom used in the treatment of viperid snake bite envenoming in Central America. The S/D treatment by 1% tri (n-butyl) phosphate (TnBP) – 1% Triton X-45 at 22–25°C was applied either on starting plasma or on purified immunoglobulins. The S/D agents were removed from both fractions by extractions with oil. S/D-treated plasma was subjected to caprylic acid precipitation to purify the immunoglobulins. Products were formulated, sterile-filtered, and filled into 10-mL vials, stored at 5±3°C, and subjected to routine quality controls, SDS-PAGE, determination of anti-Bothrops asper venom antibody titre by ELISA, in vivo B. asper venom-neutralization potency tests, and safety test, comparatively with an antivenom manufactured by caprylic acid fractionation without S/D treatment. Results indicate that these conditions of S/D treatment on purified immunoglobulin yielded an antivenom of high turbidity that induced weight loss in animals. In contrast, antivenom fractionated from the S/D-treated plasma had physico-chemical and biological characteristics indistinguishable from those of the non-S/D-treated antivenom. S/D treatment of horse plasma may be considered to increase the viral safety of antivenoms. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10451056
- Volume :
- 37
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Biologicals
- Publication Type :
- Academic Journal
- Accession number :
- 44120327
- Full Text :
- https://doi.org/10.1016/j.biologicals.2009.05.003