Resolution of the Mexican transgene detection controversy: error sources and scientific practice in commercial and ecological contexts.

The article focuses on the use of genetic identification (ID) to detect positive blind samples and prove that polymerase chain reaction (PCR) is a reliable method in determining specific DNA sequences in Mexican landrace maize. It notes that the PCR amplification of transgenic sequences in maize backgrounds tend to produce relatively faint bands of variable intensity but this should be considered due to various reasons, including the gradual activation of Taq polymerase. Accordingly, the sequence diversity among the events of the detected transgenic transformation was confirmed by the sequencing data provided by the researchers. Furthermore, an enzyme-linked immunosorbent assay (ELISA) targeted at detecting recombinant proteins in maize shows that transgenes are present in the maize.