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Expression of the Th1-Specific Cell-Surface Protein Tim-3 Increases in a Murine Model of Atopic Asthma.
- Source :
-
Journal of Asthma . Nov2009, Vol. 46 Issue 9, p872-877. 6p. - Publication Year :
- 2009
-
Abstract
- T-cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3) is preferentially expressed on Th1-helper type T-cells and functions to repress the Th1-mediated immune response. However, the role of Tim-3 during the inflammatory pathogenesis of asthma remains unclear. This study determines the expression level of Tim-3 in CD4+ T-cells within the peripheral blood and bronchoalveolar lavage fluid (BALF) isolated from a murine model of atopic asthma and explores the potential role of Tim-3 during the inflammatory response. Mice were randomly divided into normal control, asthma day 1, and asthma day 7 groups, and peripheral blood T lymphocytes and BALF cells were collected. The ratio of Tim-3+/CD4+ cells among the total CD4+cell populations from peripheral blood and BALF was determined by flow cytometry, and the expression of the Tim-3 mRNA was determined by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). In contrast with the normal control group, the ratio of Tim-3+/CD4+:CD4+ cells and the level of Tim-3 mRNA in both the peripheral blood T lymphocytes and BALF cells among the asthma day 1 and asthma day 7 groups were significantly increased ( p < 0.01), and those in the asthma day 7 group were higher than the asthma day 1 group ( p < 0.05). There was also a positive correlation between the ratio of Tim-3+/CD4+:CD4+ detected in BALF and that the ratio detected in peripheral blood T lymphocytes (r = 0.84, p < 0.01). Therefore, the expression of Tim-3 is increased in CD4+ T-cells following airway challenge and likely affects asthma-induced inflammation by repressing the Th1-mediated immune response. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ASTHMATICS
*ANIMAL models in research
*T cells
*LUNG disease diagnosis
*LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 02770903
- Volume :
- 46
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Journal of Asthma
- Publication Type :
- Academic Journal
- Accession number :
- 45117991
- Full Text :
- https://doi.org/10.3109/02770900903199953