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Durable, region-specific protein patterning in microfluidic channels
- Source :
-
Biomaterials . Jan2010, Vol. 31 Issue 2, p315-320. 6p. - Publication Year :
- 2010
-
Abstract
- Abstract: We present a straightforward, accessible method to covalently pattern proteins in poly(dimethyl siloxane) (PDMS) microchannels. Our approach includes (i) region-specific photografting of a layer of poly(acrylamide) (PAAm) and (ii) bioconjugation of PAAm with a desired protein. The method produces symmetric protein patterns on all channel walls, which have high specificity and pattern fidelity, are compatible with a variety of geometries and exhibit excellent longevity under shear stresses of up to 1dyn/cm. We demonstrate the generality of the method by creating multi-protein gradients within microfluidic microchannels and by in-situ patterning of islands of multiple proteins. Protein activity was observed by the digestion of BODIPY–casein using channels patterned with trypsin. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01429612
- Volume :
- 31
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 45134327
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2009.09.040