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Diverse backbone-cyclized peptides via codon reprogramming.
- Source :
-
Nature Chemical Biology . Dec2009, Vol. 5 Issue 12, p888-890. 3p. 2 Diagrams. - Publication Year :
- 2009
-
Abstract
- We report a methodology for the ribosomal synthesis of backbone-cyclized peptides involving genetic code reprogramming to introduce one or more nonproteinogenic amino acids. Expression of linear peptides bearing a cysteine-proline dipeptide sequence followed by glycolic acid results in self-rearrangement to a C-terminal diketopiperadine-thioester, which non-enzymatically generates a cyclized peptide. We demonstrate the ribosomal synthesis of several naturally occurring backbone-cyclized peptides and a library based on a bicyclic scaffold, and we identify bioactive sequences by screening and deconvolution. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15524450
- Volume :
- 5
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Nature Chemical Biology
- Publication Type :
- Academic Journal
- Accession number :
- 45164158
- Full Text :
- https://doi.org/10.1038/nchembio.259