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Influence of Test Duration on the Sensitivity of the Two-bottle Choice Test.
- Source :
-
Chemical Senses . Nov2002, Vol. 27 Issue 9, p759-768. 10p. - Publication Year :
- 2002
-
Abstract
- The long-term two-bottle choice test is commonly used as a simple screen to examine the acceptance of taste solutions by rodents. As part of an investigation of factors influencing the sensitivity of the two-bottle choice test, we determined the extent to which test duration influenced test sensitivity. C57BL6/J and 129X1/SvJ mice received four series of eight two-bottle tests, with each test lasting 1, 2, 4 or 6 days. Each series involved sequential tests with water, 2 mM saccharin, 5 and 50 mM citric acid, 30 and 300 μM quinine hydrochloride, 75 mM NaCl and 10% ethanol. There were significant differences between the strains in intake of saccharin, 5 and 50 mM citric acid, NaCl and ethanol in 4 and 6 day tests, but only saccharin and ethanol in 2 day tests, and 5 mM citric acid and ethanol in 1 day tests. To compare the sensitivity of the tests, we developed an analytical approach based on the comparison of deviations of individual 129X1/SvJ mice from the C57BL6/J strain mean. Our results suggest that to discriminate between strains or treatments when using `standard' laboratory conditions and methods, 1 day tests are generally inadequate and 2 day tests are useful only if large effects are anticipated. Tests lasting 4 or 6 days are more sensitive, but conducting 6 day tests provides little additional benefit and sometimes is detrimental relative to conducting 4 day tests. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CITRIC acid
*QUININE
*ETHANOL
*SACCHARIN
*LABORATORY rodents
Subjects
Details
- Language :
- English
- ISSN :
- 0379864X
- Volume :
- 27
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Chemical Senses
- Publication Type :
- Academic Journal
- Accession number :
- 45304189
- Full Text :
- https://doi.org/10.1093/chemse/27.9.759