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Selective Transmission of R5 HIV-1 over X4 HIV-1 at the Dendritic Cell-T Cell Infectious Synapse Is Determined by the T Cell Activation State.
- Source :
-
PLoS Pathogens . Jan2009, Vol. 5 Issue 1, p1-12. 12p. 6 Graphs. - Publication Year :
- 2009
-
Abstract
- Dendritic cells (DCs) are essential antigen-presenting cells for the induction of T cell immunity against HIV. On the other hand, due to the susceptibility of DCs to HIV infection, virus replication is strongly enhanced in DC-T cell interaction via an immunological synapse formed during the antigen presentation process. When HIV-1 is isolated from individuals newly infected with the mixture of R5 and X4 variants, R5 is predominant, irrespective of the route of infection. Because the early massive HIV-1 replication occurs in activated T cells and such T-cell activation is induced by antigen presentation, we postulated that the selective expansion of R5 may largely occur at the level of DC-T cell interaction. Thus, the immunological synapse serves as an infectious synapse through which the virus can be disseminated in vivo. We used fluorescent recombinant X4 and R5 HIV-1 consisting of a common HIV-1 genome structure with distinct envelopes, which allowed us to discriminate the HIV-1 transmitted from DCs infected with the two virus mixtures to antigen-specific CD4+ T cells by flow cytometry. We clearly show that the selective expansion of R5 over X4 HIV-1 did occur, which was determined at an early entry step by the activation status of the CD4+ T cells receiving virus from DCs, but not by virus entry efficiency or productivity in DCs. Our results imply a promising strategy for the efficient control of HIV infection. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HIV infection transmission
*T cells
*LYMPHOCYTES
*LENTIVIRUS diseases
*HIV
Subjects
Details
- Language :
- English
- ISSN :
- 15537366
- Volume :
- 5
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- PLoS Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 45408251
- Full Text :
- https://doi.org/10.1371/journal.ppat.1000279