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MicroRNA-214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells.
- Source :
-
IUBMB Life . Nov2009, Vol. 61 Issue 11, p1075-1082. 8p. 1 Chart, 4 Graphs. - Publication Year :
- 2009
-
Abstract
- MicroRNAs are a group of endogenously expressed, single-stranded, 18–24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3′UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs. © 2009 IUBMB IUBMB Life, 61(11): 1075–1082, 2009 [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15216543
- Volume :
- 61
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- IUBMB Life
- Publication Type :
- Academic Journal
- Accession number :
- 45479489
- Full Text :
- https://doi.org/10.1002/iub.252