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Epitopes for broad and potent neutralizing antibody responses during chronic infection with human immunodeficiency virus type 1
- Source :
-
Virology . Jan2010, Vol. 396 Issue 2, p339-348. 10p. - Publication Year :
- 2010
-
Abstract
- Abstract: Neutralizing antibody (nAb) response is sporadic and has limited potency and breadth during infection with human immunodeficiency virus type 1 (HIV-1). In rare cases, broad and potent nAbs are actually induced in vivo. Identifying specific epitopes targeted by such broad and potent nAb response is valuable in guiding the design of a prophylactic vaccine aimed to induce nAb. In this study, we have defined neutralizing epitope usage in 7 out of 17 subjects with broad and potent nAbs by using targeted mutagenesis in known neutralizing epitopes of HIV-1 glycoproteins and by using in vitro depletion of serum neutralizing activity by various recombinant HIV-1 glycoproteins. Consistent with recent reports, the CD4 binding site (CD4BS) is targeted by nAbs in vivo (4 of the 7 subjects with defined neutralizing epitopes). The new finding from this study is that epitopes in the gp120 outer domain are also targeted by nAbs in vivo (5 of the 7 subjects). The outer domain epitopes include glycan-dependent epitopes (2 subjects), conserved nonlinear epitope in the V3 region (2 subjects), and a CD4BS epitope composed mainly of the elements in the outer domain (1 subject). Importantly, we found indication for epitope poly-specificity, a dual usage of the V3 and CD4BS epitopes, in only one subject. This study provides a more complete profile of epitope usage for broad and potent nAb responses during HIV-1 infection. [Copyright &y& Elsevier]
- Subjects :
- *EPITOPES
*HIV infections
*GLYCOPROTEINS
*MUTAGENESIS
*AIDS vaccines
*IMMUNOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 396
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 45658350
- Full Text :
- https://doi.org/10.1016/j.virol.2009.10.044