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Presence, distribution, and diversity of iron-oxidizing bacteria at a landfill leachate-impacted groundwater surface water interface.
- Source :
-
FEMS Microbiology Ecology . Feb2010, Vol. 71 Issue 2, p260-271. 12p. 1 Diagram, 3 Charts, 4 Graphs. - Publication Year :
- 2010
-
Abstract
- We examined the presence of iron-oxidizing bacteria (IOB) at a groundwater surface water interface (GSI) impacted by reduced groundwater originating as leachate from an upgradient landfill. IOB enrichments and quantifications were obtained, at high vertical resolution, by an iron/oxygen opposing gradient cultivation method. The depth-resolved soil distribution profiles of water content, Fe2+, and total Fe indicated sharp gradients within the top 10 cm sediments of the GSI, where the IOB density was the highest. In addition, the vertical distribution of iron-reducing bacteria at the same sampling site mirrored the IOB distribution. Clone libraries from two separate IOB enrichments indicated a stratified IOB community with clear differences at short vertical distances. Alpha- and Betaproteobacteria were the dominant phylotypes. Clones from the near-surface sediment (1–2 cm below ground surface) were dominated by members of the Bradyrhizobiaceae and Comamonadaceae; clones from the deeper sediments were phylogenetically more diverse, dominated by members of the Rhodocyclaceae. The iron deposition profiles indicated that active iron oxidation occurred only within the near-to-surface GSI sediments. The match between the iron deposition profiles and the IOB abundance profiles strongly hints at the contribution of the IOB community to Fe oxidation in this Fe-rich GSI ecosystem. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01686496
- Volume :
- 71
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- FEMS Microbiology Ecology
- Publication Type :
- Academic Journal
- Accession number :
- 47124247
- Full Text :
- https://doi.org/10.1111/j.1574-6941.2009.00797.x