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Highly sensitive and selective detection of Hg2+ using mismatched DNA and a molecular light switch complex in aqueous solution
- Source :
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Biosensors & Bioelectronics . Feb2010, Vol. 25 Issue 6, p1338-1343. 6p. - Publication Year :
- 2010
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Abstract
- Abstract: A novel “signal-on” assay for sensitive and selective detection of Hg2+ in aqueous solution based on mismatched DNA and a molecular light switch complex, [Ru(phen)2(dppz)]2+, was reported in the present work. This Hg2+ specific sensor system composed of two label-free DNA probes with eight T–T mismatches, which could form stable DNA duplexes by thymine–Hg2+–thymine (T–Hg2+–T) in the presence of Hg2+. The luminescence of [Ru(phen)2(dppz)]2+ is very weak in aqueous solution, and significant luminescence can be observed when intercalating into DNA duplexes. By monitoring Hg2+-dependent luminescence intensity enhancement, highly sensitive and selective determination of Hg2+ has been achieved. Under the optimum conditions, the luminescence intensity was proportional to the concentration of Hg2+ in the range of 1.0×10−9–1.5×10−7 M. A detection limit of 3.5×10−10 M Hg2+ was achieved using 3σ. The presence of other metal ions did not interfere with the detection of Hg2+ even at high concentrations. This approach is simple and rapid, which can be used to monitor the Hg2+ concentration in drinking water and natural media within 30min. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 25
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 47456058
- Full Text :
- https://doi.org/10.1016/j.bios.2009.10.023