Acanthopanax senticosides B ameliorates oxidative damage induced by hydrogen peroxide in cultured neonatal rat cardiomyocytes

Abstract: Acanthopanax senticosides B is a monomer of Acanthopanax senticosus saponins. Previous reports showed that Acanthopanax senticosus saponins exhibit antioxidant action and cardiac protection. However, whether Acanthopanax senticosides B has cardiac protection remains unknown. In this study, we investigated the effect of Acanthopanax senticosides B on oxidative damage induced by hydrogen peroxide (H2O2) of cardiomyocytes. As revealed from the results, when the cardiomyocytes were exposed to H2O2 for designed time and concentration, the percentage of survival cells was down significantly, the activity of lactate dehydrogenase (LDH) and the content of cellular malondialdehyde (MDA) were increased markedly. These results indicated that the cells were subjected to oxidative damage. Treatment with Acanthopanax senticosides B prior to H2O2 (100μM) exposure can increase cell viability significantly, lessen H2O2-induced cardiomyocyte morphological change, and inhibit augmentation of LDH activity in culture media and cellular MDA content markedly. Our results demonstrated that Acanthopanax senticosides B (400μg/ml and 200μg/ml) can protect cells against oxidative injury of H2O2 (100μM). Furthermore, the activities of superoxide dismutase (SOD), glutathione peroxidase, catalase and the content of reduced glutathione (GSH) of cardiomyocytes were also raised by Acanthopanax senticosides B. Taken together, the study implicated that Acanthopanax senticosides B protects cardiomyocytes against oxidative-stress injury induced by H2O2 through reduction of lipid peroxidation and enhancement of the activity of antioxidant defense. [Copyright &y& Elsevier]