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Parylene-A coated microplate for covalent immobilization of proteins and peptides
- Source :
-
Journal of Immunological Methods . Feb2010, Vol. 353 Issue 1/2, p44-48. 5p. - Publication Year :
- 2010
-
Abstract
- Abstract: Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400–500nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules. [Copyright &y& Elsevier]
- Subjects :
- *MICROPLATES
*IMMOBILIZED proteins
*POLYSTYRENE
*IMMUNOASSAY
*HORSERADISH
*PEROXIDASE
Subjects
Details
- Language :
- English
- ISSN :
- 00221759
- Volume :
- 353
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Immunological Methods
- Publication Type :
- Academic Journal
- Accession number :
- 48148705
- Full Text :
- https://doi.org/10.1016/j.jim.2009.11.015