Back to Search
Start Over
Determination of nanograms of proteins based on the amplified resonance light scattering signals of Tichromine
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . Mar2010, Vol. 75 Issue 3, p1057-1060. 4p. - Publication Year :
- 2010
-
Abstract
- Abstract: A new high-sensitivity detection of protein assay at the nanogram level is developed based on the amplified resonance light scattering signals (RLS) of Tichromine (TCM). In Walpole (NaAc–HCl) buffer (pH 4.05), TCM reacts with proteins to form large particles, which results in remarkable enhanced RLS signals characterized by three peaks at 293nm, 399nm and 553nm. Mechanistic studies showed that the enhanced RLS stems from a large complex of TCM–BSA formed for the electrostatic effect between TCM and BSA. With the enhanced RLS signals at the three wavelengths, the enhanced RLS intensity is proportional to the concentration of proteins in an appropriate range. The lowest limit of determination was 7.4ngmL−1. The proposed method was successfully applied to determine total protein in human serum samples. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 75
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 48220260
- Full Text :
- https://doi.org/10.1016/j.saa.2009.12.053