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Comparative studies on the physico-chemical properties of hemicelluloses obtained by DEAE-cellulose-52 chromatography from sugarcane bagasse
- Source :
-
Food Research International . Apr2010, Vol. 43 Issue 3, p683-693. 11p. - Publication Year :
- 2010
-
Abstract
- Abstract: Water- and alkali-soluble hemicelluloses isolated from dewaxed sugarcane bagasse were sub-fractionated on DEAE-cellulose-52 chromatography and obtained six hemicellulosic sub-fractions by eluting with water, 0.1M and 0.3M NaCl aqueous solution, respectively. Sugar composition and molecular weight analysis revealed that the lower molecular weight (14,180–43,590gmol−1) and more branches of hemicelluloses could be extracted by the hot water, which are rich in glucose, galactose, and xylose, while the higher molecular weight (75,430–138,170gmol−1) and more linear hemicelluloses were able to be dissolved into 1% NaOH aqueous solution, which are rich in xylose, principally resulting from l-arabino-(4-O-methyl-glucurono)-d-xylans. In addition, it was found that with increasing the concentration of NaCl (aqueous), the hemicellulosic sub-fractions with both higher arabinose to xylose ratio and higher molecular weight were eluted. Based on the FT-IR, sugar composition and 1H and 13C NMR comparative studies, the alkali-soluble hemicellulosic sub-fractions had a classical structure, with a backbone of β-(1→4)-linked xylosyl residue substituted with arabinose at C–2 and/or C–3 of main chain, whereas the difference may occur in the distribution of branches along the xylan backbone. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09639969
- Volume :
- 43
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Food Research International
- Publication Type :
- Academic Journal
- Accession number :
- 48220987
- Full Text :
- https://doi.org/10.1016/j.foodres.2009.10.020