Back to Search
Start Over
Surrobodies with Functional Tails
- Source :
-
Journal of Molecular Biology . Mar2010, Vol. 397 Issue 1, p352-360. 9p. - Publication Year :
- 2010
-
Abstract
- Abstract: Surrobodies 2 [2] The Surrobody and Surrobodies marks are trademarks of Sea Lane Biotechnologies, LLC. are a unique type of binding protein based on the pre-B-cell receptor (pre-BCR). The pre-BCR is transiently expressed during development of the antibody repertoire. Unlike heterotetrameric canonical antibodies that are composed of identical pairs of heavy and light chains, the pre-BCR is a heterohexameric complex composed of identical pairs of heavy chains that are each paired with a two-subunit surrogate light chain (SLC). The SLC contains nonimmunoglobulin-like peptide extensions on each of the two SLC components. This arrangement provides unique opportunities for protein engineering by functional derivatization of these nonimmunoglobulin-like tails. Here we report recombinant fusions to these tails with either a fully active cytokine or with single-chain variable fragment (scFv) domains to generate Surrobodies with unique functions or Surrobodies that are bispecific with respect to targeted binding. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00222836
- Volume :
- 397
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 48381054
- Full Text :
- https://doi.org/10.1016/j.jmb.2010.01.036