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Biosynthesis of the Putative Siderophore Erythrochelin Requires Unprecedented Crosstalk between Separate Nonribosomal Peptide Gene Clusters
- Source :
-
Chemistry & Biology . Feb2010, Vol. 17 Issue 2, p160-173. 14p. - Publication Year :
- 2010
-
Abstract
- Summary: The genome of the erythromycin-producing bacterium Saccharopolyspora erythraea contains many orphan secondary metabolite gene clusters including two (nrps3 and nrps5) predicted to govern biosynthesis of nonribosomal peptide-based siderophores. We report here the production by S. erythraea, even under iron-sufficient conditions, of a 2,5-diketopiperazine siderophore candidate we have named erythrochelin. Deletion of the nonribosomal peptide synthetase (NRPS) gene ercD within the nrps5 cluster abolished erythrochelin production. The tetrapeptide backbone of erythrochelin (α-N-acetyl-δ-N-acetyl-δ-N-hydroxyornithine-serine-δ-N-hydroxyornithine-δ-N-acetyl-δ-N-hydroxyornithine) suggests an orthodox colinear model for erythrochelin assembly. Curiously, the δ-N-acetyltransferase required for erythrochelin biosynthesis is encoded within a remote NRPS-cluster (nrps1) whose own NRPS contains an inactivating mutation. Disruption of the nrps1 gene mcd abolished erythrochelin biosynthesis, which could then be restored by addition of synthetic L-δ-N-acetyl-δ-N-hydroxyornithine, confirming an unprecedented example of functional crosstalk between nrps clusters. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10745521
- Volume :
- 17
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Chemistry & Biology
- Publication Type :
- Academic Journal
- Accession number :
- 48405360
- Full Text :
- https://doi.org/10.1016/j.chembiol.2010.01.011