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Analysis of the 22-NBD-cholesterol transfer between liposome membranes and its relation to the intermembrane exchange of 25-hydroxycholesterol
- Source :
-
Colloids & Surfaces B: Biointerfaces . May2010, Vol. 77 Issue 1, p117-121. 5p. - Publication Year :
- 2010
-
Abstract
- Abstract: The transfer of 22-NBD-cholesterol (22-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3-ol) between two liposome membranes was quantitatively analyzed by using the fluorescence resonance energy transfer (FRET) method. Liposomes labeled with both 22-NBD-cholesterol and a rhodamine-labeled phosphatidylethanolamine (Rh-DHPE) were used as donor liposomes, and the 22-NBD-cholesterol transfer from these donor liposomes to acceptor liposomes prepared from same type of phosphatidylcholine was monitored. The transfer kinetics was found to be composed of a fast and a slow phase, and all kinetic measurements could be fitted with a bi-exponential model. The results obtained indicate that the 22-NBD-cholesterol transfer kinetics between liposome membranes depends on the fluidity of the liposome used and that the curvature may affect the kinetics. Furthermore, the behavior of 22-NBD-cholesterol in lipid membrane is similar to that of the oxysterol 25-hydroxycholesterol rather than cholesterol. It is proposed that 22-NBD-cholesterol can be a useful fluorescent probe to mimic the intermembrane transfer of oxidized cholesterols like 25-hydroxycholesterol, rather than that of cholesterol itself. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09277765
- Volume :
- 77
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Colloids & Surfaces B: Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 48470536
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2010.01.002