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Atomic accuracy in predicting and designing noncanonical RNA structure.
- Source :
-
Nature Methods . Apr2010, Vol. 7 Issue 4, p291-294. 4p. 1 Chart, 2 Graphs. - Publication Year :
- 2010
-
Abstract
- We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6–20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 7
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 48847368
- Full Text :
- https://doi.org/10.1038/nmeth.1433