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Overexpression of LAPTM4B promotes growth of gallbladder carcinoma cells in vitro
- Source :
-
American Journal of Surgery . Apr2010, Vol. 199 Issue 4, p515-521. 7p. - Publication Year :
- 2010
-
Abstract
- Abstract: Background: The overexpression of LAPTM4B-35 in gallbladder carcinoma (GBC) and its clinicopathologic and prognostic significance have been previously shown. Thus, this gene may play a role in the growth of GBC cells. Methods: The pcDNA3-AE containing the complete open reading frame of LAPTM4B (lysosome-associated protein transmembrane-4β) and mock (pcDNA3) plasmids were transiently transfected into GBC-SD cells. Cell proliferation, cell cycle distribution, and protein expression were evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium assay, flow cytometry, and Western blot, respectively. Results: Cells transfected with pcDNA3-AE revealed accelerated proliferation, less serum dependence, and significant cell cycle progression compared with cells transfected with mock plasmid and parent cells. These phenotypes were accompanied by upregulated expression of C-myc, c-Fos, c-Jun, cyclin D1, and cyclin E and downregulated expression of P16 and P-27. Conclusions: LAPTM4B overexpression promotes the growth of GBC cells in vitro by regulating the expression levels of some proliferation-associated proteins. Therefore, the LAPTM4B gene might be used as a novel therapeutic target of GBC. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00029610
- Volume :
- 199
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- American Journal of Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 49124759
- Full Text :
- https://doi.org/10.1016/j.amjsurg.2009.03.031