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INFECCIONES CONCURRENTES POR DOS SEROTIPOS DEL VIRUS DENGUE DURANTE UN BROTE EN EL NOROESTE DE PERÚ, 2008.

Authors :
Mamani, Enrique
Figueroa, Dana
García, María Paquita
del Carmen Garaycochea, María
Pozo, Edwar J.
Source :
Revista Peruana de Medicina Experimental y Salud Pública. 2010, Vol. 27 Issue 1, p16-21. 6p. 1 Black and White Photograph, 1 Diagram, 3 Charts.
Publication Year :
2010

Abstract

Objetives. To establish the existence of concurrent infections by different dengue virus (DENV) serotypes in an outbreak in the Northwestern in Peru during 2008. Material and methods. 73 serum samples from patients with dengue were analyzed during an outbreak that occurred in Northwestern in Peru between May and June 2008. Molecular biology techniques were used to serotype the DENV, thus, firstly the viral RNA viral was extracted using Viral QIAamp RNA mini kit (Qiagen, Valencia, California, USA), then the viral cDNA fragments were reverse transcripted and amplified by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and the RT-Nested PCR region techniques and finally, genetic sequencing of the viral cDNA fragments were performed using the Big Dye Terminator v.3,1 kit. Results. The 73 dengue cases presented infections by different serotypes: 34 (46.6%) by DENV-3, 29 (39.7%) by DENV-1, 4 (5.5%) by DENV-4, and 6 (8.2%) concurrent infections by DENV-1 and DENV-3. The most frequent clinical manifestations observed among dengue patients were fever and headache (100%), myalgia (94.5%), ocular pain (83.6%), arthralgia (78.1%), shivers (63.0%), nausea/vomiting (38.4%), positive tourniquet test (30.1%), and rash (20.5%). All patients with concurrent infections presented light clinical course of dengue fever (Df) except one patient who had moderate hemorrhagic manifestations. Conclusion. This is the first Peruvian report of patients with concurrent infections of two DENV serotypes without severe clinical manifestations. [ABSTRACT FROM AUTHOR]

Details

Language :
Spanish
ISSN :
17264634
Volume :
27
Issue :
1
Database :
Academic Search Index
Journal :
Revista Peruana de Medicina Experimental y Salud Pública
Publication Type :
Academic Journal
Accession number :
50250595
Full Text :
https://doi.org/10.1590/S1726-46342010000100004