Assessment of genotoxic effects of benzyl derivatives by the comet assay

Abstract: In this study, different concentrations of four benzyl derivatives (benzyl alcohol, benzyl acetate, benzoic acid and benzaldehyde) used as flavour ingredients were investigated for genotoxicity in in vitro. By taking blood from two healthy people comet assay was carried on to investigate the potential health damages of benzyl derivatives. For the evaluation of genotoxic effects, the tail moment and % tail DNA in the treated chemicals were compared to the solvent control, which is distilled water. The alkaline comet assay showed significantly increased tail moment and % tail DNA at 25 and 50mM concentrations of benzyl alcohol. Benzyl acetate increased both % tail DNA and tail moment at 50mM concentrations. While % tail DNA was statistically increased at 10mM and higher concentrations, tail moment has significant difference at 10 and 25mM concentrations of benzaldehyde. Benzoic acid has apoptotic effects at the concentrations higher than 5mM, for this reason we tested concentrations less than 5mM (0.05, 0.1, 0.5, 1 and 5mM). Only the highest concentration of benzoic acid increased both tail moment and % tail DNA. [Copyright &y& Elsevier]