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Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology
- Source :
-
Enzyme & Microbial Technology . Jun2010, Vol. 46 Issue 7, p581-587. 7p. - Publication Year :
- 2010
-
Abstract
- Abstract: A novel hot spring bacterial strain was isolated and characterized from Bakreshwar, West Bengal, India. Phylogenetic analysis of partial 16 S-rRNA gene sequence (697 nucleotides) suggested that the isolate was affiliated with the family ‘Firmicutes’ and 99.8% homologous with Thermoanaerobactor thermohydrosulfuricus. A unique thermostable and high pH tolerant protein (28kDa) secreted by the bacterium was isolated whose N-terminal partial sequence (25 AA) suggested that the protein was similar to mammalian carbonic anhydrase-II. This bacterium or its unique protein both increase the compressive (>25%) and tensile (>20%) strengths, hardness and elastic modulus of cementitious material when incorporated to the cement/cement–sand mixture. The protein (named as bioremediase) possesses silica leaching activity, which is increased by calcium ions. No esterase and/or proteolytic activities are present in the protein. The use of such isolated bacterium or its protein in concrete technology develops new construction material that may be used for self-healing concrete in future. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01410229
- Volume :
- 46
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Enzyme & Microbial Technology
- Publication Type :
- Academic Journal
- Accession number :
- 50359165
- Full Text :
- https://doi.org/10.1016/j.enzmictec.2010.03.005