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Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes
- Source :
-
Microbiological Research . May2010, Vol. 165 Issue 4, p321-328. 8p. - Publication Year :
- 2010
-
Abstract
- Summary: An electrophoretic karyotype of Korean Flammulina velutipes was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The monokaryotic progenies (4019-20 and 4019-18) and a dikaryotic strain (4019-2018) had 6–8 chromosomes, with sizes ranging from 1.6 to 5.8 megabase (Mb) pairs. Among the 3 strains that were examined, strain 4019-20 resolved into at least 7 chromosomes, with a total genome size of approximately 26.7Mb. We selected several chromosome-specific genes from the cDNA library of F. velutipes using Southern hybridization analysis. In order to determine the whole genome sequence, we constructed a bacterial artificial chromosome (BAC) library of the 4019-20 strain. The BAC library comprised 3840 clones, and the insert size ranged from 60 to 228kb, with an average size of 136kb. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09445013
- Volume :
- 165
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Microbiological Research
- Publication Type :
- Academic Journal
- Accession number :
- 50696848
- Full Text :
- https://doi.org/10.1016/j.micres.2009.06.003