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Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3
- Source :
-
Plasmid . Jul2010, Vol. 64 Issue 1, p36-40. 5p. - Publication Year :
- 2010
-
Abstract
- Abstract: The complete nucleotide sequence of cryptic plasmid pXY3 isolated from Lactobacillus plantarum strain XY3 has been determined. It consisted of a 2968-bp circular molecule with a G+C content of 39%. Sequence analysis of pXY3 revealed three putative open reading frames (ORFs). Based on sequence similarity, the Rep protein shared 89% and 88% identity with Rep proteins of pLF24 and pWCFS102, respectively, which belonged to the rolling-circle replication (RCR) pMV158 family. A ssoT-like single-strand origin (sso) and a typical pMV158 family double-strand origin (dso) located upstream of the rep gene. Southern blot analysis indicated pXY3 replicate via a rolling-circle (RC) mechanism. Furthermore, the relative copy number of pXY3 was estimated to be about 97 copies per chromosome equivalent by real-time PCR. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0147619X
- Volume :
- 64
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Plasmid
- Publication Type :
- Academic Journal
- Accession number :
- 51304008
- Full Text :
- https://doi.org/10.1016/j.plasmid.2010.03.003