Back to Search
Start Over
A macrophage migration inhibitory factor-like tautomerase from Teladorsagia circumcincta (Nematoda: Strongylida).
- Source :
-
Parasite Immunology . Jul2010, Vol. 32 Issue 7, p503-511. 9p. 1 Color Photograph, 1 Black and White Photograph, 1 Diagram, 3 Graphs. - Publication Year :
- 2010
-
Abstract
- A macrophage migration inhibitory factor (MIF)-like molecule, Tci-MIF-1, was isolated from Teladorsagia circumcincta and subjected to detailed characterization. A cDNA representing Tci-mif-1 was isolated following its identification in third-stage larvae (L3)-enriched cDNA population. Sequencing of the cDNA indicated a 348-bp open reading frame (ORF) with the closest orthologue being a MIF derived from the human hookworm Ancylostoma ceylanicum. Messenger RNA (mRNA) representing the Tci-MIF-1 transcript was detected in eggs, L3 and adult stages of T. circumcincta. The transcript was also present, but to a lesser extent in fourth-stage larvae (L4). Detection of Tci-MIF-1 protein in T. circumcincta developmental stages reflected the transcript levels identified by reverse transcriptase-PCR. Using immunohistochemistry, the Tci-MIF-1 protein was shown to have a diffuse distribution in L3 tissue, and in L4 and adult stages, the protein was localized to the nematode gut. A recombinant version of Tci-MIF-1 was produced, and enzymic assays indicated that this recombinant protein and a somatic extract of L3 possessed dopachrome tautomerase activity as has been observed previously in other MIF-like molecules. Neither native, purified Tci-MIF nor recombinant Tci-MIF-1 dramatically influenced the in vitro migration of sheep monocytes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MACROPHAGES
*NEMATODES
*NUCLEIC acids
*RECOMBINANT proteins
*MESSENGER RNA
Subjects
Details
- Language :
- English
- ISSN :
- 01419838
- Volume :
- 32
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Parasite Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 51305371
- Full Text :
- https://doi.org/10.1111/j.1365-3024.2010.01215.x