Conjugation of Glucose Oxidase onto Mn-Doped ZnS Quantum Dots for Phosphorescent Sensing of Glucose in Biological Fluids.

Integrating various enzymes with nanomaterials provides various nanohybrids with new possibilities in biosensor applications. Furthermore, the enzymatic activity and stability are also improved due to the large surface area of nanomaterials. Here we report the conjugation of glucose oxidase (GOD) onto phosphorescent Mn-doped ZnS quantum dots (QDs) using l-ethyl-3-(3-dimethylaminopropy)carbodiimide(EDC)/ JV-hydroxysuccinimide (NHS) as coupling reagents for glucose biosensing based on the effective quenching of the room temperature phosphorescence (RTP) of Mn-doped ZnS QDs by the H2O2 generated from GOD-catalyzed oxidation of glucose. The obtained biocon-jugatenotonlyprovided improved enzymatic performance with Michaelis-Menten constant of 0.70 mM but also favored biological applications because the phosphorescent detection mode avoided the interference from autofluorescence and scattering light from the biological matrix. In addition, the GOD-conju-gated Mn-doped ZnS QDs showed better thermal stability in the temperature range of 20-80 °C. The GOD-Mn-doped ZnS QDs based RTP sensor for glucose gave a detection limit of 3 μM and two linear ranges from 10 /iM to 0.1 mM and from 0.1 to 1 mM. The developed biosensor was successfully applied to the determination of glucose in real serum samples without the need for any complicated sample pretreatments. [ABSTRACT FROM AUTHOR]