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Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection
- Source :
-
Carbohydrate Polymers . Aug2010, Vol. 82 Issue 1, p189-194. 6p. - Publication Year :
- 2010
-
Abstract
- Abstract: A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03–325fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572μA/fM with a response time of 12s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4°C. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01448617
- Volume :
- 82
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Polymers
- Publication Type :
- Academic Journal
- Accession number :
- 51921273
- Full Text :
- https://doi.org/10.1016/j.carbpol.2010.04.051