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Intestinal commensal bacteria promote T cell hyporesponsiveness and down-regulate the serum antibody responses induced by dietary antigen
- Source :
-
Immunology Letters . Aug2010, Vol. 132 Issue 1/2, p45-52. 8p. - Publication Year :
- 2010
-
Abstract
- Abstract: Colonization of the gut by commensal bacteria modulates the induction of oral tolerance and allergy. However, how these intestinal bacteria modulate antigen-specific T cell responses induced by oral antigens remains unclear. In order to investigate this, we used germ-free (GF) ovalbumin (OVA)-specific T cell receptor transgenic (OVA23-3) mice. Conventional (CV) or GF mice were administered an OVA-containing diet. Cytokine production by CD4+ cells from spleen (SP), mesenteric lymph nodes (MLN) and Peyer''s patches (PP) was evaluated by ELISA, as was the peripheral antibody titer. T cell phenotype was assessed by flow cytometry. CD4+ cells from the SP and MLN of CV and GF mice fed an OVA diet for 3 weeks produced significantly less IL-2 than the corresponding cells from mice receiving a control diet, suggesting that oral tolerance could be induced at the T cell level in the systemic and intestinal immune systems of both bacterial condition of mice. However, we also observed that the T cell hyporesponsiveness induced by dietary antigen was delayed in the systemic immune tissues and was weaker in the intestinal immune tissues of the GF mice. Intestinal MLN and PP CD4+ T cells from these animals also produced lower levels of IL-10, had less activated/memory type CD45RBlow cells, and expressed lower levels of CTLA-4 but not Foxp3 compared to their CV counterparts. Furthermore, GF mice produced higher serum levels of OVA-specific antibodies than CV animals. CD40L expression by SP CD4+ cells from GF mice fed OVA was higher than that of CV mice. These results suggest that intestinal commensal bacteria promote T cell hyporesponsiveness and down-regulate serum antibody responses induced by dietary antigens through modulation of the intestinal and systemic T cell phenotype. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01652478
- Volume :
- 132
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Immunology Letters
- Publication Type :
- Academic Journal
- Accession number :
- 52304861
- Full Text :
- https://doi.org/10.1016/j.imlet.2010.05.007