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Structure of the O polysaccharide and immunochemical relationships between the lipopolysaccharides of Pseudomonas syringae pathovar tomato and pathovar maculicola.
- Source :
-
European Journal of Biochemistry . Dec98 Part 1, Vol. 258 Issue 2, p657-661. 5p. 2 Charts, 1 Graph. - Publication Year :
- 1998
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Abstract
- O polysaccharides (OPS) of the lipopolysaccharides (LPS) of Pseudomonas syringae pathovar tomato GSPB 483 and pathovar maculicola IMV 381 were studied by 1H-NMR and 13C-NMR spectroscopy, including two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments. The OPS from both strains were shown to be chemically identical. Two structurally different types of repeating units (O repeats 1 and 2) were elucidated in each OPS. The minor O repeat is a pentasaccharide having the structure 2. The same structure has been reported earlier for some other OPS of P. syringae. The major O repeat is a hexasaccharide with the novel structure 1 which is distinguished from 2 by the presence of the second lateral residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc) and by a different position of substitution of one of the L-rhamnose (Rha) residues in the backbone. α-D-Fucp3NAc α-D-Fucp3NAc 1 1 ↓ ↓ 3 3 ←2)-α-L-Rhap-(1←2)-α-L-Rhap-(1←3)-α-L-Rhap-(1←3)-α-L-Rhap-(1← 1 α-D-Fucp3NAc 1 ↓ 3 ←2)-α-L-Rhap-(1←3)-α-L-Rhap-(1←3)-α-L-Rhap-(1←3)-α-L-Rhap-(1← 2 Structural and serological diversity of OPS of strains belonging to P. syringae pv. tomato and pv. maculicola and phylogenetic relatedness of the strains are discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- *POLYSACCHARIDES
*PSEUDOMONAS syringae
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 258
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 5276285
- Full Text :
- https://doi.org/10.1046/j.1432-1327.1998.2580657.x