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ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF FLUNITRAZEPAM AND 7-AMINOFLUNITRAZEPAM IN HUMAN PLASMA.
- Source :
-
Journal of Liquid Chromatography & Related Technologies . 2010, Vol. 33 Issue 14, p1381-1392. 12p. 1 Diagram, 1 Chart, 5 Graphs. - Publication Year :
- 2010
-
Abstract
- A high-throughput ultra-performance liquid chromatography (UPLC) method with UV detection was elaborated and validated for the simultaneous quantification of flunitrazepam and its major metabolite, 7-aminoflunitrazepam, in human plasma. After a simple liquid-liquid extraction with ethyl acetate, chromatographic separation was performed on a BEH C18 chromatographic column with a binary mixture of acetonitrile/10 mM potassium monohydrogen phosphate (pH = 8.5) as the mobile phase, under gradient elution (flow rate = 0.275 mL/min). The total run time of the chromatographic analysis was 4.2 min. UV detection was performed at 314 nm for flunitrazepam and at 242 nm for 7-aminoflunitrazepam. To our knowledge, this is the first reported UPLC-PDA method for the quantification of flunitrazepam and 7-aminoflunitrazepam in human plasma. The assay was applied successfully for the analysis of the selected two analytes in real human plasma samples obtained from a healthy volunteer, after ingestion of a single dose of flunitrazepam. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10826076
- Volume :
- 33
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- Journal of Liquid Chromatography & Related Technologies
- Publication Type :
- Academic Journal
- Accession number :
- 53299982
- Full Text :
- https://doi.org/10.1080/10826076.2010.489441