Back to Search
Start Over
Automated chemiluminescent dual-analyte immunoassay based on resolved immunosensing channels
- Source :
-
Talanta . Sep2010, Vol. 82 Issue 4, p1462-1467. 6p. - Publication Year :
- 2010
-
Abstract
- Abstract: A novel system of series-wound immunosensing channels (SWIC) was proposed for automated chemiluminescent (CL) dual-analyte immunoassay by immobilizing respectively different capture antibodies on the inner walls of series-wound glass channels. This system could use a single enzyme as label to perform multiplex immunoassay in one fluid way. Using α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as model analytes, the mixture including AFP, horseradish peroxidase (HRP)-labeled anti-AFP antibody, CEA and HRP-labeled anti-CEA antibody was introduced into the SWIC for carrying out the on-line incubation. Upon injection of CL substrate the CL signals from the two immunosensing channels were conveniently resolved and near-simultaneously collected with the aid of optical shutter. AFP and CEA could be rapidly assayed in the ranges of 1.0–100 and 1.0–80ng/ml with detection limits of 0.41 and 0.39ng/ml, respectively. The assay results of clinical serum samples were in an acceptable agreement with the reference values. This designed flow-through immunosensing system based on SWIC provided an automated, reusable, simple, sensitive and low-cost approach for multianalyte immunoassay. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 82
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 53333229
- Full Text :
- https://doi.org/10.1016/j.talanta.2010.07.018