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Dynamics in cytokine responses during the development of occupational sensitization to rats.
- Source :
-
Allergy . Oct2010, Vol. 65 Issue 10, p1227-1233. 7p. 3 Charts, 3 Graphs. - Publication Year :
- 2010
-
Abstract
- To cite this article: Krop EJM, van de Pol MA, Lutter R, Heederik DJJ, Aalberse RC, van der Zee JS. Dynamics in cytokine responses during the development of occupational sensitization to rats. Allergy 2010; 65: 1227–1233. Background: Occupational allergy forms an attractive model to study the development of allergic responses, as in some occupations it has a high incidence and develops quickly. In a cohort of starting laboratory animal workers, we previously found 20% sensitization to animal allergens within 2 years. Methods: We compared cellular responses of incident laboratory animal workers who developed rat-specific sensitization (cases, n = 18) during 2 years of follow-up to control animal workers matched for atopic status but without sensitization after follow-up (controls, n = 18). Practically, this is a case–control study, nested within the cohort. Rat-specific IgE antibodies were measured in sera, and allergen-specific and nonspecific cytokine responses were measured in whole blood and in isolated peripheral blood mononuclear cells. Results: Self-reported allergic symptoms were related to the presence of rat-specific IgE ( P ≤ 0.01). Cases developed a rat allergen–specific interleukin (IL)-4 response during sensitization, while controls did not show an increased IL-4 response (at visit D: 33 vs 5 IL-4 producing cells/106 cells, P < 0.001). The IL-4 response was related to the levels of rat-specific IgE in cases (visit D: rho = 0.706, P < 0.001). By contrast, allergen-specific IL-10 and interferon γ (IFNγ) responses as well as nonspecific cytokine responses were comparable between cases and controls. Conclusion: This study is the first to show the development of an allergen-specific IL-4 response in adult human subjects during allergen-specific sensitization. This IL-4 response was quantitatively associated with the development of the specific IgE antibodies. Allergen-specific or nonspecific IL-10 and IFNγ responses showed no protective effect on the development of allergic sensitization. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01054538
- Volume :
- 65
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 53418146
- Full Text :
- https://doi.org/10.1111/j.1398-9995.2009.02323.x