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Efficient in vitro labeling rabbit neural stem cell with paramagnetic Gd-DTPA and fluorescent substance
- Source :
-
European Journal of Radiology . Sep2010, Vol. 75 Issue 3, p397-405. 9p. - Publication Year :
- 2010
-
Abstract
- Abstract: Objectives: The aim of this study is to label rabbit neural stem cells (NSCs) by using standard contrast agents (Gd-DTPA) in combination with PKH26 and in vitro track them with MR imaging. Materials and methods: NSCs from prenatal brains of rabbits were cultured and propagated. Intracellular uptake of Gd-DTPA was achieved by using a non-liposomal lipid transfection reagent (Effectene) as the transfection agent. After labeling with Gd-DTPA, cells were incubated with cellular membrane fluorescent dye PKH26. The labeling effectiveness and the longevity of Gd-DTPA maintenance were measured on a 1.5T MR scanner. The influence of labeling on the cellular biological behaviors was assessed by cellular viability, proliferation and differentiation assessment. Results: The labeling efficiency of Gd-DTPA was up to 90%. The signal intensity on T1-weighted imaging and T1 values of labeled cells were significantly higher than those of unlabeled cells (P <0.05). The minimal number of detectable cells for T1-weighted imaging was 5×103. Cellular uptake of Gd-DTPA was maintained until 15 days after initially labeling. There was no significant difference in the cellular viability and proliferation between the labeled and unlabeled NSCs (P >0.05). Normal glial and neuronal differentiation remained in labeled NSCs like unlabeled NSCs. Conclusion: Highly efficient labeling NSCs with Gd-DTPA could be achieved by using Effectene. This method of labeling NSCs allows for tracking cells with MR imaging, and without alterations of cellular biological behaviors. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0720048X
- Volume :
- 75
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- European Journal of Radiology
- Publication Type :
- Academic Journal
- Accession number :
- 53790042
- Full Text :
- https://doi.org/10.1016/j.ejrad.2009.04.040