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Involvement of heme oxygenase-1 in the anti-inflammatory activity of Chrysanthemum boreale Makino extracts on the expression of inducible nitric oxide synthase in RAW264.7 macrophages
- Source :
-
Journal of Ethnopharmacology . Oct2010, Vol. 131 Issue 3, p550-554. 5p. - Publication Year :
- 2010
-
Abstract
- This study is to elucidate the involvement of anti-inflammatory heme oxygenase-1 (HO-1) in the inhibitory activity of a Chrysanthemum boreale Makino (CB) extract on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Materials and methods: Cell viability and NO assay were performed. In addition, iNOS expression was detected by Western blotting and real-time PCR. HO-1 expression was also evaluated by Western blotting, and blocking HO-1 activity on NO production was performed. Results: The CB extract at the highest concentration (100μg/ml) significantly inhibited NO production by approximately 90% and suppressed iNOS protein expression by approximately 84.8% compared to LPS-stimulated cells. Furthermore, the CB extract (100μg/ml) inhibited iNOS mRNA expression in a concentration-dependant manner and suppressed iNOS mRNA expression by 94.8%. The CB extract induced the expression of HO-1 in a dose-dependent manner, and blocking HO-1 activity abolished the inhibitory effects of the CB extract. Moreover, the addition of carbon monoxide such as tricarbonyl dichlororuthenium (II) dimmer (RuCO), a byproduct derived from heme degradation, mimicked the inhibitory action of low concentrations of CB extract. Conclusion: These results suggest that a CB extract has potent anti-inflammatory activity in RAW264.7 macrophages involving the induction of HO-1. [Copyright &y& Elsevier]
- Subjects :
- *MACROPHAGES
*KILLER cells
*MESSENGER RNA
*WESTERN immunoblotting
*HEMOGLOBINS
*MEDICINAL plants
*ETHNOPHARMACOLOGY
*ALTERNATIVE medicine
*ANALYSIS of variance
*ANIMAL experimentation
*ANTI-inflammatory agents
*BIOLOGICAL assay
*BIOLOGICAL models
*BIOPHYSICS
*ENZYMES
*RESEARCH methodology
*MICE
*NITRIC oxide
*POLYMERASE chain reaction
*PLANT extracts
*STATISTICAL significance
*REVERSE transcriptase polymerase chain reaction
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 131
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 53790330
- Full Text :
- https://doi.org/10.1016/j.jep.2010.07.030