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Expression of Interleukin-17 in Autoimmune Dacryoadenitis in MRL/lpr Mice.
- Source :
-
Current Eye Research . Oct2010, Vol. 35 Issue 10, p865-871. 7p. 1 Color Photograph, 3 Graphs. - Publication Year :
- 2010
-
Abstract
- Purpose: Th-cytokines play important roles in dacryoadenitis associated with Sjögren's syndrome (SS). The aims of this study were to (i) investigate whether interleukin (IL)-17, a newly identified Th-cytokine, participates in autoimmune dacryoadenitis of MRL/lpr mice and (ii) examine the relationships among IL-17, IL-4, and interferon (IFN)-γ in these mice. Methods: Three-month-old MRL/lpr mice with lacrimal gland inflammation and 3-month-old control BALB/c mice were used. Frozen sections of lacrimal glands and submandibular lymph nodes were stained with monoclonal antibodies to cytokines IL-17, IL-4, and IFN-γ. Concentrations of IL-17 in serum and 48-h splenocyte culture supernatants were detected by ELISA. Results: Marked lacrimal gland inflammation was observed in all MRL/lpr mice but not in any BALB/c control mice. In the lacrimal glands of MRL/lpr mice, the proportions of cells that stained were 5 ± 3% for IL-17, 32 ± 14% for IL-4, and 4 ± 2% for IFN-γ. In the submandibular lymph nodes of MRL/lpr mice, expression of IL-17 and IL-4 was significantly higher than that of IFN-γ, and all three cytokines were significantly higher in MRL/lpr mice than in BALB/c mice. Concentrations of IL-17 in both serum and splenocyte culture supernatants of MRL/lpr mice were elevated compared to BALB/c controls. Conclusions: Since expression of IL-17 is elevated in 3-month-old MRL/lpr mice, we suggest that it plays a role in the pathophysiology of autoimmune dacryoadenitis in these mice. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02713683
- Volume :
- 35
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Current Eye Research
- Publication Type :
- Academic Journal
- Accession number :
- 53843642
- Full Text :
- https://doi.org/10.3109/02713683.2010.497600