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A Conserved Active-site Threonine Is Important for Both Sugar and Flavin Oxidations of Pyra nose 2-Oxidase.
- Source :
-
Journal of Biological Chemistry . 3/26/2010, Vol. 285 Issue 13, p9697-9705. 9p. - Publication Year :
- 2010
-
Abstract
- Pyranose 2-oxidase (P20) catalyzes the oxidation by O2 of n-glucose and several aldopyranoses to yield the 2-ketoaldoses and H2O2. Based on crystal structures, in one rotamer conformation, the threonine hydroxyl of Thr169 forms H-bonds to the flavin-N5/04 locus, whereas, in a different rotamer, it may interact with either sugar or other parts of the P20∙sugar complex. Transient kinetics of wild-type (WT) and Thr169 → S/N/ G/A replacement variants show that D-Glc binds to T169S, T169N, and WT with the same Kd (45-47 mM), and the hydride transfer rate constants (kred) are similar (15.3-9.7 s-1 at 4°C). kred of T169G with n-glucose (0.7 s-1, 4 °C) is significantly less than that of WT but not as severely affected as in T169A (kred of 0.03 s-1 at 25 °C). Transient kinetics of WI and mutants using n-galactose show that P20 binds n-galactose with a one-step binding process, different from binding of n-glucose. In T169S, T169N, and 1169G, the overall turnover with n-Gal is faster than that of WT due to an increase of kred. In the crystal structure of T169S, Sen69 Oγ assumes a position identical to that of Oγ1 in Ihri69 in T169G, solvent molecules may be able to rescue H-bonding. Our data suggest that a competent reductive half-reaction requires a side chain at position 169 that is able to form an H-bond within the ES complex. During the oxidative half-reaction, all mutants failed to stabilize a C4a-hydroperoxyflavin intermediate, thus suggesting that the precise position and geometry of the Thr169 side chain are required for interme- diate stabilization. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OXIDASES
*OXIDATION
*GLUCOSE
*FLAVINS
*HYDRIDES
*GALACTOSE
*HYDROGEN bonding
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 285
- Issue :
- 13
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 53897082
- Full Text :
- https://doi.org/10.1074/jbc.M109.073247