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VEGF and BMP-6 enhance bone formation mediated by cloned mouse osteoprogenitor cells.
- Source :
-
Growth Factors . Oct2010, Vol. 28 Issue 5, p306-317. 12p. 5 Color Photographs, 1 Diagram, 3 Charts, 4 Graphs. - Publication Year :
- 2010
-
Abstract
- New strategies such as combined utilization of growth factors may provide a better treatment for difficult fractures. We have demonstrated enhanced angiogenesis and osteogenesis through the actions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-6 (BMP-6) on the osteogenic differentiation of a cloned mouse osteoprogenitor cell in vitro and ectopic bone formation in vivo. Human VEGF and BMP-6 genes expressed together produced a significant increase in alkaline phosphatase activity, expression of the RunX2 and osteocalcin genes and mineralization. Microcomputed tomographic analysis of subcutaneous implants consisting of cells transfected with VEGF and BMP-6 cDNA and delivered on a 3D poly (lactic-co-glycolic acid) scaffold confirmed the additive effects between VEGF and BMP-6. Ectopic bone formation in the VEGF plus BMP-6 group was greatest compared to that in either VEGF or BMP-6 alone. This is the first study that demonstrates osteogenesis in vitro and in vivo through the additive effects of VEGF and BMP-6. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08977194
- Volume :
- 28
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Growth Factors
- Publication Type :
- Academic Journal
- Accession number :
- 54098909
- Full Text :
- https://doi.org/10.3109/08977194.2010.484423