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Expression profiles of genes within a subregion of chicken major histocompatibility complex B in spleen after Marek's disease virus infection.
- Source :
-
Poultry Science . Oct2010, Vol. 89 Issue 10, p2123-2129. 7p. - Publication Year :
- 2010
-
Abstract
- Major histocompatibility complex has previously been shown to influence the resistance of chicken to Marek's disease virus (MDV). However, little is known about expression of other genes in the MHC-I and II pathway after MDV infection. This study aimed at investigating 8 immune-related genes in the MHC core region that affects host responses to MDV. Spleens of infected and age-matched uninfected chickens were removed at 4, 7, 14, 21, and 28 d postinfection for gene expression detection using real-time PCR. Different expression patterns of MHC-I and II pathway genes were observed in the spleen. In the MHC-I pathway, the expression of transporter of antigen protein 1 (TAP1), transporter of antigen protein 2 (TAP2), and transporter of antigen protein-binding protein (TAPBP) genes was significantly increased in the spleen of MDV-infected than that of uninfected chickens. It indicated that host antivirtis responses were generated to enhance antigen presentation. However, MHC-II pathway genes showed contrarary trends. Classical MHC-II β chain major gene (BLB2) and nonclassical class II genes [DM a chain gene (IpMA), DM β chain gene-i (DMB1), and DM β chain gene-2 (DMB2)] had consistent lower transcripts in spleens of MDV-infected than that of uninfected chicken's, which reflected that MDV interfered with multiple components of the MHC-II pathway. Overall, expression of most genes in the MHC core region was altered; moreover, the genes in endogenous and exogenous antigen presentation pathways had different expression patterns in the spleen after MDV infection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00325791
- Volume :
- 89
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Poultry Science
- Publication Type :
- Academic Journal
- Accession number :
- 54290643
- Full Text :
- https://doi.org/10.3382/ps.2010-00919