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Zebrafish mast cells possess an FcɛRI-like receptor and participate in innate and adaptive immune responses
- Source :
-
Developmental & Comparative Immunology . Jan2011, Vol. 35 Issue 1, p125-134. 10p. - Publication Year :
- 2011
-
Abstract
- Abstract: We previously identified a zebrafish mast cell (MC) lineage and now aim to determine if these cells function analogously in innate and adaptive immunity like their mammalian counterparts. Intraperitoneal (IP) injection of compound 48/80 or live Aeromonas salmonicida resulted in significant MC degranulation evident histologically and by increased plasma tryptase compared with saline-injected controls (p =0.0006, 0.005, respectively). Pre-treatment with ketotifen abrogated these responses (p =0.0004, 0.005, respectively). Cross-reactivity was observed in zebrafish to anti-human high-affinity IgE receptor gamma (FcɛRIγ) and IgE heavy chain-directed antibodies. Whole mount in situ hybridization on 7-day embryos demonstrated co-localization of cpa5, a MC-specific marker, with myd88, a toll-like receptor adaptor, and zebrafish FcɛRI subunit homologs. Zebrafish injected IP with matched dinitrophenyl-sensitized mouse (anti-DNP) IgE and DNP-BSA or trinitrophenyl-sensitized mouse (anti-TNP) IgE and TNP-BSA demonstrated increased plasma tryptase compared with mismatched controls (p =0.03, 0.010, respectively). These results confirm functional conservation and validate the zebrafish model as an in vivo screening tool for novel MC modulating agents. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 35
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 54483477
- Full Text :
- https://doi.org/10.1016/j.dci.2010.09.001