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Development of a PCR-based SNP marker system for effective selection of kernel length and kernel elongation in rice.
- Source :
-
Molecular Breeding . Dec2010, Vol. 26 Issue 4, p735-740. 6p. 1 Diagram, 1 Chart, 2 Graphs. - Publication Year :
- 2010
-
Abstract
- Kernel length in rice ( Oryza sativa L.) is controlled by various quantitative trait loci of which GS3 is the most important, being responsible for 80-90% of the variation in kernel length. A mutation in the second exon of this gene has been reported to be associated with maximum variations in the kernel length. We have developed a simple PCR-based marker system named DRR-GL which targets the functional nucleotide polymorphism at GS3. This marker system has the advantages that it is easy to use, saves time and cost, and is amenable for large-scale marker-assisted selection for the trait of kernel length. Validation of this marker in a segregating population and 152 rice varieties, which includes 30 elite basmati varieties, reveals its effective co-segregation and association with the traits of kernel length as well as kernel elongation after cooking. We recommend utilization of this simple, low-cost marker system in breeding programs targeted at improvement of key rice grain quality traits, kernel length and kernel elongation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13803743
- Volume :
- 26
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Molecular Breeding
- Publication Type :
- Academic Journal
- Accession number :
- 54589073
- Full Text :
- https://doi.org/10.1007/s11032-010-9492-3