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Molecular detection of poisonous mushrooms in different matrices.
- Source :
-
Mycologia . May/Jun2010, Vol. 102 Issue 3, p747-754. 8p. 3 Charts, 1 Graph. - Publication Year :
- 2010
-
Abstract
- Amanita phalloides, Lepiota cristata, Lepiota brunneoincarnata and Inocybe asterospora are among the most important species responsible of mushroom poisoning in northern Italy. A real time PCR method for the identification of samples containing DNA from each of these species was developed. To test specificity all protocols were applied on DNA extracted from various mushroom species; sensitivity was assessed performing serial dilutions on all samples; versatility of the protocols was evaluated performing tests on DNA extracted from different matrices. The protocols showed high sensitivity (32 ng dried mushroom), high specificity and sensitive detection of DNA extracted from difficult samples, including pasta with mushroom, cooked mushrooms and gastric aspirates. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BASIDIOMYCETES
*POISONOUS mushrooms
*DNA
*PLANT species
*MATRICES (Mathematics)
Subjects
Details
- Language :
- English
- ISSN :
- 00275514
- Volume :
- 102
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Mycologia
- Publication Type :
- Academic Journal
- Accession number :
- 54728331
- Full Text :
- https://doi.org/10.3852/09-124