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Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T‐cell lymphomas.
- Source :
-
Clinical & Experimental Dermatology . Jul2000, Vol. 25 Issue 5, p423-431. 9p. - Publication Year :
- 2000
-
Abstract
- Various enzymatic or mechanical methods have been proposed in the past to dissociate cells from different solid tissues. An automated mechanical disaggregation device (Medimachine™) has recently been proposed. Unfortunately, most of these techniques are associated with a high cellular damage and a low cell recovery and are difficult to apply to skin biopsies. In this paper, we propose a combined enzymatic and mechanical method based on Medimachine™, useful for the isolation of skin infiltrating T-lymphocytes from small cutaneous biopsies. As this method is easy and allows for a more correct qualitative and quantitative cytofluorimetric analysis of the lymphocyte subsets, it may be useful in the immunophenotyping of cutaneous T-cell lymphomas. [ABSTRACT FROM AUTHOR]
- Subjects :
- *T cells
*LYMPHOMAS
*SKIN biopsy
*IMMUNOPHENOTYPING
Subjects
Details
- Language :
- English
- ISSN :
- 03076938
- Volume :
- 25
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 5481015
- Full Text :
- https://doi.org/10.1046/j.1365-2230.2000.00680.x