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Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA.
- Source :
-
PLoS ONE . 2009, Vol. 4 Issue 6, p1-10. 10p. 1 Diagram, 6 Graphs. - Publication Year :
- 2009
-
Abstract
- Gametophytic self-incompatibility (GSI) allows plants to block fertilization by haploid pollen whose S-allele constitution matches one of the two S-alleles in the diploid styles. GSI in Solanum chacoense requires a stylar S-RNase, first secreted from cells of the transmitting tract then imported into incompatible (self) pollen tubes. However, the molecular mechanisms allowing compatible pollen to evade S-RNase attack are less clear, as compatible pollen tubes also import S-RNase. Using styles of the same age and size in order to lower the degree of inter-style variability in S-RNase levels, we observe reduction of up to 30% of the total non-self stylar S-RNase in vivo during compatible crosses, whereas no degradation of self S-RNases is detected. This marked difference in stylar S-RNase levels dovetails with measurements of pollen-specific Lat52 mRNA, which decreases four-fold in incompatible compared to compatible crosses. Unexpectedly, we also find evidence for a reciprocal signaling mechanism from compatible pollen to the cells of the transmitting tract that results in a roughly threefold decrease in S-RNase transcript levels. These findings reveal a previously unsuspected feedback loop that may help reinforce the compatible reaction. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 4
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 55981875
- Full Text :
- https://doi.org/10.1371/journal.pone.0005774