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Histidine 90 Function in 4-Chlorobenzoyl-Coenzyme A Dehalogenase Catalysis.

Authors :
Wenchai Zhang
Yansheng Wei
Lusong Luo
Taylor, Kimberly L.
Guang Yang
Dunaway-Mariano, Debra
Source :
Biochemistry. 11/13/2001, Vol. 40 Issue 45, p13474. 8p. 7 Diagrams, 1 Chart, 4 Graphs.
Publication Year :
2001

Abstract

Examines the contribution of the active site Histidine 90 (His90) to the 4-chlorobenzoyl-coenzyme A dehalogenase catalysis. Replacement of His90 by glutamine by site-directed mutagenesis; Use of X-ray crystallographic analysis; Evaluation of the kinetic binding and catalytic turnover rate.

Details

Language :
English
ISSN :
00062960
Volume :
40
Issue :
45
Database :
Academic Search Index
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
5657301
Full Text :
https://doi.org/10.1021/bi0114426