Histidine 90 Function in 4-Chlorobenzoyl-Coenzyme A Dehalogenase Catalysis.

Authors :: Wenchai Zhang
Yansheng Wei
Lusong Luo
Taylor, Kimberly L.
Guang Yang
Dunaway-Mariano, Debra
Source :: Biochemistry. 11/13/2001, Vol. 40 Issue 45, p13474. 8p. 7 Diagrams, 1 Chart, 4 Graphs.
Publication Year :: 2001
Abstract: Examines the contribution of the active site Histidine 90 (His90) to the 4-chlorobenzoyl-coenzyme A dehalogenase catalysis. Replacement of His90 by glutamine by site-directed mutagenesis; Use of X-ray crystallographic analysis; Evaluation of the kinetic binding and catalytic turnover rate.