Back to Search
Start Over
Development of Lu-DOTA-anti-CD20 for radioimmunotherapy.
- Source :
-
Journal of Radioanalytical & Nuclear Chemistry . Jan2011, Vol. 287 Issue 1, p199-209. 11p. - Publication Year :
- 2011
-
Abstract
- Rituximab was successively labeled with Lu-lutetium chloride. Lu chloride was obtained by thermal neutron flux (4 × 10 n cm s) of natural LuO sample with a specific activity of 2.6-3 GBq/mg. The macrocyclic bifunctional chelating agent, N-succinimidyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA-NHS) was prepared at 25 °C using DOTA, N-hydroxy succinimide (NHS) in CHCl. DOTA-rituximab was obtained by the addition of 1 mL of a rituximab pharmaceutical solution (5 mg/mL, in phosphate buffer, pH 7.8) to a glass tube pre-coated with DOTA-NHS (0.01-0.1 mg) at 25 °C with continuous mild stirring for 15 h. Radiolabeling was performed at 37 °C in 24 h. Radio-thin layer chromatography showed an overall radiochemical purity of >98% at optimized conditions (specific activity = 444 MBq/mg, labeling efficacy; 82%). The final isotonic Lu-DOTA-rituximab complex was checked by gel electrophoresis for structure integrity control. Radio-TLC was performed to ensure that only one species was present after filtration through a 0.22 μm filter. Preliminary biodistribution studies in normal rats were carried out to determine complex distribution of the radioimmunoconjugate up to 168 h. The biodistribution data were in accordance with other antiCD20 radioimmunoconjugates already reported. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LUTETIUM
*RADIOIMMUNOTHERAPY
*RITUXIMAB
*CHELATES
*HEAT flux
*NEUTRONS
*ACETIC acid
Subjects
Details
- Language :
- English
- ISSN :
- 02365731
- Volume :
- 287
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Radioanalytical & Nuclear Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 56615901
- Full Text :
- https://doi.org/10.1007/s10967-010-0676-4