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Low doses of killed parasite in CpG elicit vigorous CD4+ T cell responses against blood-stage malaria in mice.
- Source :
-
Journal of Clinical Investigation . Aug2010, Vol. 120 Issue 8, p2967-2977. 12p. 7 Graphs. - Publication Year :
- 2010
-
Abstract
- Development of a vaccine that targets blood-stage malaria parasites is imperative if we are to sustainably reduce the morbidity and mortality caused by this infection. Such a vaccine should elicit long-lasting immune responses against conserved determinants in the parasite population. Most blood-stage vaccines, however, induce protective antibodies against surface antigens, which tend to be polymorphic. Cell-mediated responses, on the other hand, offer the theoretical advantage of targeting internal antigens that are more likely to be conserved. Nonetheless, few of the current blood-stage vaccine candidates are able to harness vigorous T cell immunity. Here, we present what we believe to be a novel blood-stage whole-organism vaccine that, by combining low doses of killed parasite with CpG-oligodeoxynucleotide (CpG-ODN) adjuvant, was able to elicit strong and cross-reactive T cell responses in mice. Our data demonstrate that immunization of mice with 1,000 killed parasites in CpG-ODN engendered durable and cross-strain protection by inducing a vigorous response that was dependent on CD4+ T cells, IFN-gamma, and nitric oxide. If applicable to humans, this approach should facilitate the generation of robust, cross-reactive T cell responses against malaria as well as antigen availability for vaccine manufacture. [ABSTRACT FROM AUTHOR]
- Subjects :
- *VACCINES
*MALARIA
*IMMUNE response
*T cells
*BLOOD
*PARASITES
*ANTIGEN-antibody reactions
*RESEARCH
*IMMUNIZATION
*ANIMAL experimentation
*INTERLEUKIN-2
*RESEARCH methodology
*IMMUNOMODULATORS
*MEDICAL cooperation
*EVALUATION research
*NUCLEOTIDES
*INTERFERONS
*COMPARATIVE studies
*IMMUNITY
*MICE
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 00219738
- Volume :
- 120
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Journal of Clinical Investigation
- Publication Type :
- Academic Journal
- Accession number :
- 56682884
- Full Text :
- https://doi.org/10.1172/JCI39222