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Aspirin differentially regulates endotoxin-induced IL-12 and TNF-alpha production in human dendritic cells.
- Source :
-
Scandinavian Journal of Rheumatology . Dec2001, Vol. 30 Issue 6, p346-352. 7p. 12 Graphs. - Publication Year :
- 2001
-
Abstract
- <bold>Objective: </bold>In the development of autoimmune diseases, dendritic cells (DC) play critical roles. Here, we examined the effect of aspirin on lipopolysaccharide (LPS)-induced DC activation.<bold>Methods: </bold>The monocyte-derived DC were established. The cytokine production was measured by ELISA, reverse transcriptase/polymerase chain reaction, or intracellular staining analyzed by flow cytometry. The expression of cell surface molecules was determined by flow cytometry.<bold>Results: </bold>Aspirin inhibited LPS-induced DC maturation and costimulatory molecules expression. Aspirin, at therapeutic concentrations, also decreased LPS-induced IL-12 and IL-10 production. In contrast, the LPS-induced TNF-alpha production was enhanced by aspirin. The differential effects of aspirin on IL-12 and TNF-alpha production may not be due to down-regulation of cyclooxygenase activities.<bold>Conclusion: </bold>The various effects of aspirin on LPS-stimulated DC may influence the understanding of the diverse immunomodulatory mechanisms of this anti-inflammatory drug. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ASPIRIN
*DENDRITIC cells
*PHYSIOLOGY
*BIOCHEMISTRY
*CELL culture
*COMPARATIVE studies
*DOCUMENTATION
*DOSE-effect relationship in pharmacology
*INTERLEUKINS
*PHENOMENOLOGY
*RESEARCH methodology
*MEDICAL cooperation
*NUCLEOTIDES
*POLYMERASE chain reaction
*REFERENCE values
*RESEARCH
*TUMOR necrosis factors
*EVALUATION research
*REVERSE transcriptase polymerase chain reaction
*LIPOPOLYSACCHARIDES
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03009742
- Volume :
- 30
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Scandinavian Journal of Rheumatology
- Publication Type :
- Academic Journal
- Accession number :
- 5691760
- Full Text :
- https://doi.org/10.1080/030097401317148543